The Reserve is located in the southeastern slopes of the central mountains in the department of Antioquia, Colombia. Sharing boundaries between the municipalities of Puerto Triunfo, San Luis and Sonson, 40 km from the source of the river between the Tigre and Osa peaks (Sonson), and 57 km from its mouth on the Magdalena River. The reserve is located 3 hours from Medellin by the Medellin-Bogota highway at 152 km and 5 Hours from Bogota at 264 km.

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Location: south-eastern slope of the Cordillera Central, department of Antioquia
Area: 250 hectare
Height: River: 350 meters*. Cones karstic: 600 meters.
Weather: Hot, humid tropical
Vegetation: moist tropical forest. Karstic (which grows on rocks marble and limestone).

* Meters above sea level